Genom

Introduction

Genom is a tool for calculating the number of the genes in synteny blocks between two prokaryotic  genomes. It uses the table generated by GeneOrder4.01.
GeneOrder4.0 is a tool for performing comparative analysis of gene order and synteny in genomes. It presents genome comparisons between two organisms as a table, listing the order of similar genes. Using this table in Excel, we generate such a .csv file which serves as input file for Genom. The application takes those synteny blocks into account where at least two genes are in the same order. It counts  these genes and as a result we get the number of genes with the same order between two prokaryotic genomes.

Installing Genom

1.
Download the Genom.zip file
2.
Unpack and run Genom_Setup.exe
3.
Follow the on-screen instructions

Using Genom

Setting up the input file


The input file should be in .CSV format. The file contains two columns. It could be created using the output file obtained by GeneOrder4.0
Open the Output file of GeneOrder4.0 in Excel
Delete columns C and D
Delete row one
Select entire columns A and B
Use command Replace
Find what: (*)       Replace with: leave blank
We get data in two columns
Save the data as a .csv file
This is the input file for Genom

Using the program

Use the button with letter G after opening the application to start the program
Select a created csv file
Using buttons “convert it”, “analize it”, “look at the result” the Genom calculates the number of genes in synteny blocks between two prokaryotic genomes
Video tutorial
1Mahadevan P. and Seto D. 2010. Rapid pair-wise synteny analysis of large bacterial genomes using web-based GeneOrder4.0. BMC Research Notes 3:41.
Designed by Nagy Dénes, http://www.infoden.in.rs